Abstract: 【Objective】 Study on the influence of different key factors in the production of miniature potatoes. 【Methods】 The test-tube seedlings of potato detoxification seedling Chaohe 15 was taken as the test material, the three-factor split-area design of substrate, cutting density and the number of times of fertilizers were adopted to study the effects of various factors and their interactions on the potato miniature potato multiplication, and the method of the subordinate function was used to make a comprehensive evaluation of the production effect of various treatment combinations. 【Results】 In the two-factor interaction, substrate type×cuttings density had the greatest effect on yield traits, with highly significant differences in the number of large potatoes, effective potato yield, large potato yield, and total yield; substrate type×fertilizer number only had a significant effect on the number of single-planted potatoes and the number of effective potatoes; and cuttings density×fertilizer number had a non-significant effect on each yield trait. In the three-factor interaction, except for the significant difference in the number of large potatoes, the differences in the other indicators did not reach the significant level. 【Conclusion】 The comprehensive evaluation of the affiliation function shows that the A₂B₂C₃ combination has the largest affiliation value, and the combination of substrate of vermiculite: grass charcoal as 1:1 mixed substrate, plant spacing of 5 cm×8 cm, and the 4 times of fertilization has the largest average affiliation function value (0.806), of which the plant height affiliation function value is the best among all combinations, with the yield of effective potatoes 0.990, the yield of large potatoes 0.903, and stem thickness 0.792, which is the optimal combinations for the production of the original seed of Chaohe 15.