Abstract: 【Objective】 The aim of this study is to compare the low temperature tolerance of spring wheat seeds during germination by low temperature stress,It has provided basic data for the low temperature tolerance quality screening and planting utilization of spring wheat, and also laid a theoretical foundation for the low temperature tolerance gene mining and new germplasm creation. 【Methods】 Cold tolerance coefficient, membership function and cluster analysis were used to evaluate the low temperature tolerance of 33 spring wheat seeds during germination. 【Results】 Under low temperature stress, 9 indexes related to seed germination and seedling growth characteristics of spring wheat varieties showed a downward trend. Based on the comprehensive cold tolerance coefficient and membership function, the 33 varieties of spring wheat were divided into four different low temperature tolerance types, namely, strong low temperature tolerance, medium low temperature tolerance, weak low temperature tolerance and low temperature sensitivity. 【Conclusion】 The selection of the strong low temperature tolerance variety Xinchun 6 and the low temperature sensitivity variety Xinchun 49.