Abstract:
【
Objective 】The germplasm resources of drought tolerant
Agropyron cristatum were screened by PEG simulated water stress.【
Methods 】The seeds of 11
A. cristatum germplasm resources collected were used as the testing materials to evaluate the effects of different Polyethylene glycol (PEG-6000) concentration on six seed germination indexes (relative germination rate, relative germination potential, relative germination index, relative vitality index, relative seedling height and relative root length), and drought resistance of these materials was was comprehensively evaluated by using the membership function method.【
Results 】The results showed that PEG simulated drought stress had significant effects on the germination stage of 11
A. cristatum germplasm resources, and there were significant differences among the materials, but the indexes showed a decreasing trend with the increase of PEG concentration. Low concentration of drought stress had a certain promotion on germination indexes of A
1, A
8 and A
9, especially on
RGR,
RGI and
RVI. The germination indexes of 11
A. cristatum germplasm resources was inhibited with the increase of PEG stress concentration, and the inhibition effect on
RSH and
RRL was stronger, when the concentration reached 25%, the A
3 and A
4 indexes were completely inhibited. Correlation analysis showed that there was a significant correlation between the six indicators, and the correlation coefficient was 0.831 to 0.981,
RGP has the highest correlation with
RGI. The germination indexes of 11
A. cristatum germplasm resources were evaluated by membership function method and with
D value range of 0.387,7 to 0.504,5, and the drought resistance of A1 and A9 was better than those of other materials.【
Conclusion 】There are differences in the indexes of the germplasm resources at the germination stage of 11
A. cristatum germplasm resources under drought stress with the increase of PEG-6000 concentration, and the drought resistance is as follows: A
1>A
9>A
6>A
2>A
7>A
5>A
8>A
10>A
11>A
4>A
3.