PEG模拟干旱胁迫下11份野生冰草种质资源萌发期抗旱性评价

Drought resistance evaluation of 11 wild Agropyron cristatum germplasm at germination stage under PEG simulated stress

  • 摘要:目的 】通过PEG模拟水分胁迫筛选出耐旱冰草种质资源。【 方法 】收集11份野生冰草为试验材料,分析不同浓度的聚乙二醇(PEG-6000)模拟干旱胁迫对其种子相对发芽势(PGR)、相对发芽率(RGP)、相对发芽指数(RGI)、相对活力指数(RVI)、相对苗高(RSH)和相对根长(RRL)的影响,并采用隶属函数法对抗旱性进行综合评价。【 结果 】PEG模拟干旱胁迫对11份野生冰草种质资源萌发期各指标均有影响,且材料间差异明显,但各项指标均随着PEG浓度的增加呈下降趋势。低浓度(5%)的干旱胁迫对A1、A8和A9各项萌发指标具有一定的促进作用,尤其是对RGR,RGIRVI。随着PEG处理浓度的增加,11份野生冰草种质资源的各项萌发指标均受到了不同程度的抑制,尤其是对RSHRRL抑制作用较强,当浓度达到25%时,A3和A4各项指标均被完全抑制。6个萌发指标间存在极显著正相关关系,相关系数在0.831~0.981,RGPRGI的相关性最高。11份野生冰草种质资源的D值范围在0.387 7~0.504 5,其中,A1和A9的抗旱性优于其他材料。【 结论 】随着PEG-6000 浓度的增加,11份野生冰草种质资源萌发期各个指标均表现出明显的差异,其抗旱性强弱依次为A1>A9>A6>A2>A7>A5>A8>A10>A11>A4>A3

     

    Abstract:Objective 】The germplasm resources of drought tolerant Agropyron cristatum were screened by PEG simulated water stress.【 Methods 】The seeds of 11 A. cristatum germplasm resources collected were used as the testing materials to evaluate the effects of different Polyethylene glycol (PEG-6000) concentration on six seed germination indexes (relative germination rate, relative germination potential, relative germination index, relative vitality index, relative seedling height and relative root length), and drought resistance of these materials was was comprehensively evaluated by using the membership function method.【 Results 】The results showed that PEG simulated drought stress had significant effects on the germination stage of 11 A. cristatum germplasm resources, and there were significant differences among the materials, but the indexes showed a decreasing trend with the increase of PEG concentration. Low concentration of drought stress had a certain promotion on germination indexes of A1, A8 and A9, especially on RGR, RGI and RVI. The germination indexes of 11 A. cristatum germplasm resources was inhibited with the increase of PEG stress concentration, and the inhibition effect on RSH and RRL was stronger, when the concentration reached 25%, the A3 and A4 indexes were completely inhibited. Correlation analysis showed that there was a significant correlation between the six indicators, and the correlation coefficient was 0.831 to 0.981, RGP has the highest correlation with RGI. The germination indexes of 11 A. cristatum germplasm resources were evaluated by membership function method and with D value range of 0.387,7 to 0.504,5, and the drought resistance of A1 and A9 was better than those of other materials.【 Conclusion 】There are differences in the indexes of the germplasm resources at the germination stage of 11 A. cristatum germplasm resources under drought stress with the increase of PEG-6000 concentration, and the drought resistance is as follows: A1>A9>A6>A2>A7>A5>A8>A10>A11>A4>A3.

     

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