Abstract: 【 Objective 】To screen and mine the microbial species resources by analyzing the diversity and community composition of soil bacteria in the radiation-contaminated area. which provides a theoretical basis for the comprehensive mining and utilization of the microbial resources in radiation-contaminated areas. 【 Methods 】Soil samples were randomly collected from three sites in the high radiation area of a radiation-contaminated area in northwest China, and the composition of soil bacterial communities in the area was analyzed by using Illumina NovaSeq high-throughput sequencing technology; the bacteria within the soil were isolated by various screening methods, molecularly identified by using 16S rRNA, and their functional properties were preliminarily studied. 【 Results 】The results of high-throughput sequencing showed that the bacterial domains within the soil samples involved 30 phyla and 384 genera, with the dominant phyla being Firmicutes (52.15%), Proteobacteria (30.17%), Bacteroidota (10.42%) and Actinobacteriota (4.94%). Actinobacteriota, 4.94%). Planococcus (17.45%), Exiguobacterium (13.65%), Bacillus (6.97%) and Pontibacter (6.78%) were the dominant genera. Culturable methods were used to isolate 277 bacterial strains which belonged to 51 genera and 97 species. The dominant bacteria genera were Microvirga (24.91%), Streptomyces (16.25%), Pontibacter (7.22%), Cellulomonas (5.78%), etc. Ten potential new species were isolated, and majority of the obtained strains had strong salinity tolerance and functional enzyme-producing activities. 【 Conclusion 】The diversity of soil bacteria in radiation-contaminated areas is relatively rich.