Abstract: 【Objective】 To study the difference of milk yield and lactation regularity of Comparative analysis of lactation patterns of Xinjiang Donkey in Eastern Xinjiang Region and Xinjiang Donkey in Southern Xinjiang Region. 【Methods】 In this study, 65 healthy Xinjiang Donkey in Eastern Xinjiang Region were selected from half house feeding and half grazing. 70 farmed Xinjiang Donkey in Southorn Xinjiang whose lactation curve was fitted by inverse polynomial model, cubic model and sixth-degree polynomial model to explore the difference of lactation regularity of donkeys in different areas of Xinjiang. The optimal regression model was established based on body size traits and average monthly milk yield, which was used as the prediction model of milk level under semi-grazing and semi-house feeding conditions. 【Results】 The results showed that the sixth-degree polynomial model had the best fitting effect on the lactation curve of Xinjiang donkeys. The lactation peak was longer in eastern Xinjiang, and the average daily milk yield and peak milk yield of Xinjiang Donkey in Eastern Xinjiang Region were better than that of Xinjiang Donkey in Southorn Xinjiang. The regression model of body size and milk yield in eastern Xinjiang could be used as a prediction model of lactation level under semi-grazing and semi-house feeding condition 【Conclusion】 The lactation performance of semi-herding and semi-herding Xinjiang Donkey in Eastern Xinjiang Region is better than that of the donkeys in southern Xinjiang.