Abstract: 【Objective】 The study aims to explore the effects of applying bio-organic fertilizer on soil nutrient, enzyme activity and quality of Lycium barbarum L.. Through field experiment, the effects of different fertilizer application on soil fertility and soil enzyme activity and fruit quality of Lycium barbarum L. were analyzed finally. 【Methods】 Taking Ningqi 7 as the experimental material, conventional fertilization was control (CK), conventional fertilization + bioorganic fertilizer 2 kg (T₁) per plant, There were five treatments: conventional fertilizer + biological organic fertilizer 4 kg (T₂) per plant, conventional fertilizer + biological organic fertilizer 6 kg (T₃) per plant and conventional fertilizer + biological organic fertilizer 8 kg (T₄) per plant. 【Results】 Compared with conventional fertilization (CK), the soil fertility and soil enzyme activity were significantly increased after bio-organic fertilizer application (P < 0.05). The content of ascorbic acid, protein, total sugar and flavonoids in Lycium barbarum L. treated with bio-organic fertilizer was significantly higher than that treated in CK, and the effect of treatment T₃ was the best, which increased by 15.60%, 89.33%, 28.96% and 41.67% compared with that of CK. Compared with CK, the yield of Lycium barbarum L. was increased by 31.81% in treatment T₂ at the beginning of fruit stage, and the yield of Lycium barbarum L. was increased by 109.47% and 105.49% with treatment T₃ at the beginning of fruit stage and the peak of summer fruit stage. 【Conclusion】 Bio-organic fertilizer can improve soil fertility and soil enzyme activity to a certain extent, and the quality of Lycium barbarum L., too. The effect of bio-organic fertilizer on the quality of Lycium barbarum L. is the best, which can significantly improve the quality of Lycium barbarum.