Abstract: 【Objective】 This project aims to explore the effects of different planting row spacing on the production and water-saving efficiency of spinach and optimize the efficient and water-saving planting mode of facility spinach. 【Methods】 Mexico 2 spinach was taken as the test material, a field experiment was conducted in a vinyl house, and three kinds of row spacing was set for drill planting to study the effects of row spacing of 10 cm (T₁), 15 cm (T₂) and 20 cm (T₃) on yield, dry matter quality, water consumption and water use efficiency of spinach with uniform sowing (CK) as the control. 【Results】 The results showed that equal row spacing seeding significantly promoted the growth and yield of spinach in facilities.Compared with CK, the yield under T₁, T₂ and T₃ treatments increased by 4.39%, 6.34% and 10.04%, respectively.Among them, the yield and water use efficiency of spinach under T₃ treatment were significantly higher than those under T₁ and T₂ treatment.T₃ treatment not only promoted the dry matter accumulation of spinach, but also showed the lowest daily water consumption intensity during the harvest period, thus improving the water use efficiency.Compared with CK, the dry matter quality of spinach plants under T₃ treatment increased by 10.05%, and the population yield increased by 10.04%.Water consumption during the whole growth period decreased by 18.84%; Water use efficiency increased by 26.52%. 【Conclusion】 In summary, in the process of spinach production, the 20 cm row spacing drill planting mode is the most beneficial to improve the yield and water use efficiency of facility spinach, so it is strongly recommended to be popularized and applied.