Abstract: 【Objective】 This study aims to analyze the content of soluble solids in the center flesh of muskmelon by Genetic analysis and QTL mapping in the hope of finding out candidate genes associated with soluble solids in the heart of C. achyum fruits. 【Methods】 Six generations population (P₁, P₂, F₁, B₁, B₂, and F₂) were created using the high-sugar material P₁ and the low-sugar material P₂ as parents. A mixed major gene plus polygene inheritance model was used to analyze soluble solids content in the center flesh of six generations population. Based on the F₂ population, individual plants with higher and lower fruit soluble solids content were selected to construct two pools to locate the soluble solids content of muskmelons by using the BSA method. 【Results】 Muskmelon soluble solids conformed to the E-1 (MX2-ADI-AD) genetic model, with epistatic effects dominating the 2 main gene pairs, followed by dominant and additive effects. Two QTLs were located in chromosome 5 (827066 bp-109953bp) and chromosome 8 (11316600 bp-11729324 bp), with interval sizes of 0.27Mb and 0.41 Mb, respectively, and contained a total of 50 candidate genes. Six candidate genes related to soluble solids content of melon were screened and obtained (MELO3C014619, MELO3C014617, MELO3C014596, MELO3C014594, MELO3C019077 and MELO3C019089). 【Conclusion】 Muskmelon soluble solids conforms to the E-1 (MX2-ADI-AD) genetic model, two QTLs related to soluble solid content of muskmelon are mapped on chromosomes 5 and 8 by BSA method, and six candidate genes related to soluble solid content of muskmelon screened out.