Abstract: 【Objective】 To explore the function of the terrestrial cotton C2H2-type zinc finger protein gene GhZFP8 in the response to Verticillium wilt in cotton in the hope of laying a theoretical foundation for mining cotton disease resistance genes. 【Methods】 The cotton gene GhZFP8 was obtained by homologous cloning and its biological function explored. Bioinformatics methods were used to analyze the physicochemical properties of the gene, while the VIGS vector for the gene was constructed and transformed into cotton by Agrobacterium-mediated method to preliminarily test the disease resistance function of GhZFP8. 【Results】 The open reading frame (ORF) of GhZFP8 was 789 bp, encoded as a hydrophilic protein containing 262 amino acids, a relative molecular weight was 28.12 kD, a basic amino acid with an isoelectric point of 8.16, a lipid index of 59.31, and an average hydrophobicity of-0.718. The absence of a signal peptide in the GhZFP8 protein predicted that GhZFP8 was a non-transmembrane protein. GhZFP8 protein revealed that it had 2 ZnF-C2H2 structural domains. Its secondary structure prediction revealed that the GhZFP8 protein contained 6 α-helices and 4 β-folds. GhZFP8 gene silenced plants showed relatively reduced resistance to Verticillium wilt. 【Conclusion】 The GhZFP8 gene plays a positive regulatory role in cotton resistance to verticillium wilt.