玉米杂交种耐低温评价及指标筛选

Evaluation and screening of low temperature tolerance index of maize hybrids

  • 摘要: 【目的】 选用大田玉米杂交种,研究耐低温能力,筛选关键鉴定指标及耐低温能力强的杂交种,为玉米精准早播提供品种及评价和鉴定耐低温能力提供方法参考。 【方法】 选用36份玉米杂交种为试材,在大田环境下,设置1个常温和2个低温试验,调查测定田间低温和常温条件下出苗势、出苗率、出苗指数及平均出苗时间,共获取8个耐低温鉴定指标。应用主成分分析、聚类分析和逐步回归分析等统计方法,筛选耐低温出苗能力评价指标,引入综合得分值评价玉米杂交种耐低温出苗能力。 【结果】 随着温度的降低,出苗率、出苗势及出苗指数随之降低,出苗时间随之延长。获得2个综合指标累计贡献率为77.965%,逐步回归分析建立方程并筛选出5个关键指标可以决定综合得分D值的大部分变异,最后基于D值聚类分析,将36个杂交种分为4类,筛选出耐低温出苗能力强的品种有6份。 【结论】 筛选出可推广种植的耐低温能力强的杂交种6个,分别是航研58、和育187、新引KWS9384、新引KWS3376、华美1号、丰田14;筛选出常温出苗势、低温出苗势、低温出苗率、常温出苗指数和低温出苗指数5个关键指标,可用于玉米杂交种耐低温出苗能力的高效预测评价。

     

    Abstract: 【Objective】 This study aims to select the field maize hybrids for the identification of low temperature resistance,and the hybrids with strong low temperature resistance in the hope of providing variety reference for accurate early sowing and for evaluation and identification of low temperature resistance. 【Methods】 36 maize hybrids were selected as test materials.Under the real field environment, one normal temperature test and two low temperature tests were set up to investigate and measure the emergence potential, emergence rate, emergence index and average emergence time under low temperature and normal temperature conditions, and calculate the relative values.A total of 8 identification indexes of low temperature tolerance were obtained.Using the statistical methods of principal component analysis, cluster analysis and stepwise regression analysis, the evaluation indexes of low temperature seedling emergence tolerance were screened, and the comprehensive score was introduced to evaluate the low temperature seedling emergence tolerance of maize hybrids. 【Results】 With the decrease of temperature, the emergence rate, emergence potential and emergence index decreased, and the emergence time increased.Principal component analysis obtained that the cumulative contribution rate of the two comprehensive in dicators was 77.965%.Stepwise regression analysis established an equation and selected five key indicators, which could determine most of the variation of the comprehensive score D value.Finally, based on D-value cluster analysis, 36 hybrid varieties were divided into four categories, and 6 varieties with strong resistance to low temperature emergence were selected. 【Conclusion】 6 hybrids with strong low temperature tolerance were selected, which were Hangyan 58, Heyu 187, Xinyin KWS9384, Xinyin KWS3376, Huamei 1 and Fengtian 14.Five key indexes, including NEP, LEP, LER, NEI and LEI, were selected for efficient prediction and evaluation of low temperature emergence tolerance of maize hybrids.

     

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