Abstract: 【Objective】 To explore the characteristics of selenium uptake and transport in rice under the conditions of the local climate and main varieties in southern Xinjiang, and to clarify the effect of interaction between selenium fertilizer type and concentration on selenium content in rice grains in the hope of providing theoretical basis for the planting and production of selenium-enriched rice in southern Xinjiang. 【Methods】 The main rice variety Xindao 11 in Aksu, southern Xinjiang was used as the material, and the selenium(Se) fertilizer of nano-Se, sodium selenite and organic Se were applied to set up four spraying concentrations of 2.5, 5, 10 and 20 mg/L, then the effects of foliar spraying of Se fertilizer on Se uptake and transport in rice plants were studied. 【Results】 The results showed that different Se fertilizer treatments significantly increased the Se content in rice.The Se content in grains, polished rice, leaves, and stems increased with the increase of Se fertilizer concentration.The Se-enriched effect of three types of Se fertilizers on rice was: organic Se > sodium selenite > nano-Se.When spraying nano-Se fertilizer, most of the Se accumulated in rice husk and rice bran, and the Se content in polished rice increased not too much; while spraying organic Se or sodium selenite significantly increased the Se content in grain or milled rice. 【Conclusion】 In summary, 20 mg/L organic Se or sodium selenite has the best effect on Se biofortification of rice, and the Se content of polished rice is increased to 0.88 and 0.72 mg/kg.