Abstract: 【Objective】 To explore the identification index of early drought tolerance of rice and to screen the germplasm resources of rice drought tolerance. 【Methods】 The drought resistance of 31 rice varieties during germination was identified by 0%, 15% and 20% PEG-6000 solution. 【Results】 The root length, bud length, root number and germination rate of different rice varieties decreased with the increase of PEG concentration, and 20% PEG was determined as the identification concentration of drought tolerance of rice during germination. The 31 varieties were divided into three categories by systematic cluster analysis: the first category was best drought-resistant variety at germination stage, with 4 cultivars; the second group consisted of 14 medium drought-resistant varieties during germination, the third group consisted of 13 varieties with poor drought resistance during germination. Only one comprehensive factor was extracted by principal component analysis, and the cumulative contribution rate was 74.689%. The relative shoot length, relative root length, relative root number and relative germination rate were significantly positively correlated with the average total membership function value. These four traits were identified as drought resistance identification indicators at germination stage. 【Conclusion】 Four rice materials 23H25, 23H5, 23H20 and 23H16 with strong drought tolerance during germination have been selected by means of average total membership function and cluster analysis.