Abstract: 【Objective】 To screen a strain with high yield of non-starch polysaccharide enzyme four fungi in the hope of laying a foundation for the production of non-starch polysaccharide enzyme by solid-state fermentation of licorice residue. 【Methods】 Four kinds of fungi (Aspergillus niger, Trichoderma reesei, Phanerochaete chrysosporium and Trichoderma koningii) were primarily screened by Congo red method and filter paper strip disintegration test, and the CMC enzyme, filter paper enzyme and xylanase activities were determined by shake flask fermentation of residue.The strain with the highest enzyme activity was fermented with licorice residue in solid state to verify its enzyme production effect. 【Results】 Through the preliminary screening, it was found that all four strains of fungi had the ability to produce cellulase and xylanase.The antagonistic effects between Aspergillus Niger and other three strains was found by plate confrontation test.According to the results of enzyme activity determination, Phanerochaete chrysosporium and Trichoderma koningii produced the highest cellulase than the other two fungi, and the CMC enzyme activity reached 2.10 and 2.31 IU/mL on the fourth day of fermentation.The highest xylanase activity produced by Aspergillus Niger through shake flask re-screening fermentation could reach 97.46 IU/mL. However, the xylanase activity of Phanerochaete chrysosporium and Trichoderma koningii could reach 131.707 IU/mL at the highest. 【Conclusion】 The growth cycle of non-starch polysaccharide activity produced by mixed solid-state fermentation of licorice residue with Phanerochaete chrysosporium and Trichoderma koningii is more stable and the yield is higher, therefore, licorice residue, as an agricultural by-product, has the potential to become a low-cost substrate for enzyme production in the future.