Abstract: 【Objective】 In order to grasp the taxonomic status of Aksu wild Boletus, clarify its nutritional composition and analyze the main polysaccharide functional structure. 【Methods】 Using morphological and molecular biology methods to identify wild Boletus, analyze the nutritional components and minerals in Boletus for detection. The crude polysaccharide of Boletus pumila was extracted using water extraction and alcohol precipitation method, and purified using DEAE-52 cellulose column chromatography. Then, its monosaccharide composition was analyzed using high-pressure hydraulic chromatography. 【Results】 The wild Boletus from Aksu belongs to the order Agaricularia, family Boletus, and genus Boletus, and is identified by molecular identification as Leccinum duriusculum. Its main nutrients are sugars and proteins, accounting for 57.57% and 27.32% respectively. Three polysaccharide components were obtained through column chromatography combined with medium pressure chromatography system separation: LD-1, LD-2, and LD-3. Among them, LD-1 had the highest extraction rate of 78.8% and had strong water solubility. Infrared spectroscopy showed that it contained carbohydrate compounds with the highest proportion of glucose. 【Conclusion】 Wild Boletus in Aksu region is rich in polysaccharides and has strong water holding capacity. This result can provide scientific reference for the in-depth development and utilization of wild Boletus polysaccharides.