Abstract: 【Objective】 The objective of this project is to study the relationship between the growth and development of millet and dry matter accumulation under drought stress in different growth stages and to elucidate the effects of drought stress on the growth and yield of millet. 【Methods】 Three newly introduced millet varieties (lines) were used as experimental materials, and the water irrigation amount was artificially controlled, and drought stress treatment was carried out at different growth stages of millet to explore the changes of millet growth and yield traits under drought stress at different stages. 【Results】 (1) Drought stress had a significant effect on the agronomic traits of millet at heading stage, among which the plant height of 07607 decreased by 10.2%, the plant height and spike length of Nenxuan No. 10 decreased by 10.9% and 16.0%, respectively, and the spike length and stem base diameter of Baogu 22 decreased by 22.8% and 14.6%, respectively. (2) Drought stress at heading stage had significant effects on SPAD value, leaf area and dry matter accumulation of millet. (3) Drought at heading stage had a significant effect on plant growth and yield, and the yield was 19.6%, 37.5% and 18.4% lower than that of the control, respectively.Irrigation amount was significantly positively correlated with aboveground dry weight, biomass and yield at 0.001 level. (4) The drought resistance of different grain millet varieties (lines) was Baogu 22 > Ji 07607 > Nenxuan 10. 【Conclusion】 The heading stage is a sensitive period for millet to drought stress, and sufficient soil moisture should be ensured during this stage to ensure the increase of millet yield.