Abstract: 【Objective】 To investigate the effects of urease inhibitor N-butyl thiophosphoryl triamine (NBPT) and nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on nutrient uptake and yield in cotton. 【Methods】 Using field experiments, the tested crops were cotton Xinluzao 64 and Lumianyan 24. Four treatments were set up: no nitrogen fertilizer (N₀), nitrogen fertilizer (N₃₀₀), nitrogen fertilizer+urease inhibitor (N₃₀₀+NBPT), and nitrogen fertilizer+nitrification inhibitor (N₃₀₀+DMPP). 【Results】 The results showed that compared to N₀, all nitrogen application treatments significantly increased cotton biomass and promoted the absorption of nutrient nitrogen, phosphorus, and potassium by cotton plants. The order of nitrogen fertilizer utilization efficiency from high to low is N₃₀₀+DMPP>N₃₀₀+NBPT>N₃₀₀. Applying nitrogen fertilizer and urease/nitrification inhibitors can significantly increase cotton yield. The Lumianyan 24 had better nutrient absorption, nitrogen utilization efficiency, and higher yield than Xinluzao 64, but its biomass was significantly lower than that of Xinluzao 64. 【Conclusion】 Adding urease and nitrification inhibitors can promote the absorption and utilization of nutrients by cotton plants, improve nitrogen fertilizer utilization efficiency, and ultimately effectively increase cotton yield.