Abstract: 【Objective】 To analyze the effects of different selenium concentrations on Morchella mycelium, and to master the optimal formula of selenium-enriched medium in the hope of providing technical support for the further development of selenium-enriched products. 【Methods】 Morchella sextelatam was used as selenium enriched carrier and sodium selenite as selenium source and the effects of different selenium concentrations on the growth trend, mycelial density and mycelial morphology of Morchella sextelatam on different media were systematically analyzed. 【Results】 In liquid shake culture, when the concentration of selenium ≤ 20 mg/L, the mycelial morphology no obvious change, and the promoting effect on The mycelial growth of Morchella sextelatam with the increase of concentration, and the dry weight at 20 mg/L.The culture medium, selenium concentration ≤ 20 mg/L had no effect on the mycelial growth, mycelial density and mycelial morphology of Morchella sextelatam.the original mediumthe concentration of selenium ≤ 20 mg/L, there was no significant change in mycelial density and mycelial morphology, and it promoted the growth of Morchella sextelatam, which was the strongest at 10 mg/L.On the cultivation mediumselenium concentration of 20 mg/L the strongest promoting effect on the mycelium of Morchella sextelatam, and no significant change in mycelium density and mycelium morphology. 【Conclusion】 Sodium selenite is the best selenium source for Morchella sextelatam cultivation when concentration is 10~20 mg/L.