不同水盐生境下小叶碱蓬叶绿素荧光参数特征及其对土壤因子的响应

Characteristics of chlorophyll-fluorescence parameters of Suaeda microphylla Pall.and their responses to soil factors in different water-salt habitats

  • 摘要: 【目的】 研究干旱区盐生植物光合响应机理,为土壤盐渍化生物修复技术提供理论和科学支撑。 【方法】 以新疆艾比湖湿地国家级自然保护区典型盐生植物小叶碱蓬(Suaeda microphylla Pall.)为研究对象,选取保护区内高水高盐(生境Ⅰ)、低水中盐(生境Ⅱ)和高水中盐(生境Ⅲ)三种生境类型,测定其小叶碱蓬叶绿素荧光参数,分析三种生境下小叶碱蓬叶绿素荧光参数特性变化,研究三种生境下土壤因子对小叶碱蓬叶绿素荧光特性的影响。 【结果】 三种生境下小叶碱蓬各叶绿素荧光参数存在显著差异(P<0.05)。其中FoFmNPQ随着生境的变化逐渐升高,最大值出现在生境Ⅲ(142.95、609.42和1.65),最小值出现在生境Ⅰ(87.6、358.06和0.96)。Fv'/Fm'呈先升高再下降的趋势,最大值出现在生境Ⅱ(0.53),最小值出现在生境Ⅲ(0.39);FoFmNPQ呈极显著正相关(P<0.01),其中Fm的相关系数最大,为0.99;筛选出FoFmNPQ三个与逆境胁迫相关性较好的叶绿素荧光参数,其公因子方差为0.947、0.969、0.824;盐分、速效磷和pH对小叶碱蓬叶绿素荧光参数变异起到了较好的解释,其解释量为17.2%、24.2%、13.5%,而水分未起解释作用。 【结论】 在高盐生境下,小叶碱蓬叶片PSⅡ的结构和生理状态受到损伤,光合作用受阻,最终植物生长受到抑制。

     

    Abstract: 【Objective】 This project aims to study the photosynthetic response mechanism of halophyte in arid region in order to provide theoretical and scientific support for soil salinization bioreremediation technology. 【Methods】 In this paper, the typical halophyte Suaeda microphylla Pall.in the National Nature Reserve was studied and three types of habitats:high water salt(habitat Ⅰ)salt(habitat Ⅱ), low water and high salt water(habitat Ⅲ)were selected to measure and analyze its Suaeda microphylla Pall.chlorophyll fluorescence parameters under three habitat Suaeda microphylla Pall.characteristics of chlorophyll fluorescence parameters, thus clarifying the effects of soil factors on the fluorescence characteristics of the three habitats. 【Results】 The results showed that there were significant differences in chlorophyll fluorescence parameters among the three habitats(P<0.05).Fo, Fm and NPQ gradually increased with the change of habitat, with the maximum values in the habitat Ⅲ(142.95, 609.42 and 1.65)and the minimum values in the habitat Ⅰ(87.6, 358.06 and 0.96).Fv'/Fm' increased first and then decreased, the maximum value occurs in environment Ⅲ(0.53),the minimum value appears in scenario Ⅱ(0.39), respectively; Fo was positively correlated with Fm and NPQ(P<0.01), the correlation coefficient of Fm was the largest, which was 0.99; Three key chlorophyll fluorescence parameters, Fo, Fm and NPQ, were screened out by principal component analysis, and their common factor variances were 0.947, 0.969 and 0.824; Salt, available phosphorus and pH could explain 17.2%, 24.2% and 13.5% of the variation of chlorophyll fluorescence parameters, while water did not. 【Conclusion】 In conclusion, in the high-salt habitat, the structure and physiological state of leaves PSⅡ were damaged, photosynthesis was blocked, and plant growth was inhibited eventually.

     

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