新疆茄子绒菌斑病原菌的分离及鉴定

Isolation and identification of the Passalora nattrassii HR-1 from aubergine in Xinjiang

  • 摘要: 【目的】 分离与鉴定设施茄子上新发现的1种病害,为该病害的精准防治提供科学依据。 【方法】 试验设在新疆巴音郭楞蒙古自治州和硕县采用随机取样法,调查设施茄子上新发现病害的田间发生危害情况,采用组织分离法、柯赫氏法则、形态学观察和分子生物学方法鉴定该病害。 【结果】 菌株HR-1是该病害的病原菌。菌株HR-1与已报道的灰毛茄钉孢(Passalora nattrassii)同源性高达99.0%以上,菌株HR-1(MW362742.1)与P.nattrassii处于同一分支。 【结论】 菌株HR-1为灰毛茄钉孢(P.nattrassii),新疆设施茄子叶部新发现的病害为茄子绒菌斑病。

     

    Abstract: 【Objective】 The authors identified one new disease on aubergine in facility eggplant in Heshuo County, Mongolian Autonomous Prefecture of Bayingolin, Xinjiang, and identified the species of the disease, hoping to provide a scientific basis for accurate control of the disease. 【Methods】 Random sampling was used to investigate and clarify the damage caused by the disease in the field, and the new disease of aubergine was identified using tissue isolation, Koch's rule, morphological observations and molecular biology to determine the taxonomic status of the pathogen. 【Results】 The Koch's Law results indicated that strain HR-1 was the causal agent of the disease.Strain HR-1 was more than 99.0% homologous with the reported Passalora nattrassii.Strain HR-1(MW362742.1)was in the same branch as P.nattrassii. 【Conclusion】 The strain HR-1 was identified as P.nattrassii, a newly discovered disease of eggplant foliage in Xinjiang.

     

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