Abstract: 【Objective】 To explore the influence of ⁶⁰Co-γ radiation on the mortality rate, antioxidant enzyme activity and other related physical and chemical indexes of annual cuttings of different fig varieties, and determine the best mutagenic dosage.This study has provided a scientific theoretical basis for the future use of ⁶⁰Co-γ radiation mutagenesis to improve the genetic diversity of fig, create new germplasm and cultivate new excellent varieties. 【Methods】 The annual dormant branches of Brunswick and Zhonghuaziguo were used as test materials, and ⁶⁰Co-γ radiation as the irradiation source, different dosages(0, 30, 45, 60, 75 Gy)for radiation treatment were set to observe the changes in the death rate and enzyme activity of branches and leaves caused by irradiation. 【Results】 With the increase of radiation dosage, the survival rate of cutting seedlings of the two varieties showed a downward trend, and the indexes of plant height, stem diameter, internode length, leaf length, leaf width and branch number showed a downward trend, while the difference of leaf length-width ratio was not significant. The activities of SOD, POD, CAT and other enzymes of Zhonghuaziguo varieties showed a trend of increasing first and then decreasing, reaching the peak at the dosage of 45 and 60 Gy, respectively, MDA content showed a trend of increasing all the time, and H₂O₂ content showed a trend of increasing first and then decreasing. The activities of SOD, POD and CAT enzymes of Brunswick varieties decreased first and then increased with the increase of radiation dose. The content of MDA and H₂O₂ basically kept rising. 【Conclusion】 Radiation leads to slow growth of fig cutting seedlings, dwarf plant height, coarse and thin stem and leaves, smaller leaves and fewer branches. It is preliminarily determined that the appropriate half lethal dose of Zhonghuaziguo is 56 Gy, and that of Brunswick is 70 Gy. The radiation tolerance of Brunswick is higher than that of Zhonghuaziguo.