Abstract: 【Objective】 To study the tolerance characteristics of three species and 6 strains of entomopathogenic nematodes to temperature and salt concentration, in order to screen the strains with good tolerance to abiotic stress and determine their pathogenicity to Galleria mellonella larvae.to lay a related research foundation for the application of biological control of insect pests in the future.At the same time, the inhibitory effect of symbiotic bacteria on S.aureus, Salmonella and E.coli was studied in order to provide more new antagonistic bacteria resources for antagonistic pathogenic microorganisms. 【Methods】 The total number of nematodes was counted by dilution method, the reproductive ability of different nematodes was analyzed, and the heat tolerance and salt tolerance of nematodes were analyzed by water bath heating method and salt concentration gradient method, respectively.the pathogenicity of different doses to the Galleria mellonella larvae was determined by gradient dilution method, and the inhibitory effect of symbiotic supernatant on pathogens in different time periods was determined by coating method and drilling method. 【Results】 A strain of Steinernema-carpocapsae XJ-94 with strong comprehensive ability of reproduction and tolerance was screened from six species of nematodes.The number of nematodes produced by each large wax borer was 4.96 × 10⁴ IJs/larva, and the average number of nematodes per gram of wax borer larvae was 1.24 × 10⁴ IJs/g.After treatment at 40℃ for 5 hours, the survival rate of nematode XJ-94 was 33%, and the other strains were dead.At 17% salt concentration, 68% of the nematodes survived after treatment for 3 hours.The nematode had high pathogenicity to the Galleria mellonella larvae, and the corrected mortality of nematode doses of 160 IJs/larva and 200 IJs/larva reached 100% at 48 h.The fermentation supernatant of Xenorhabdus nematophila XJ-94 symbiotic bacteria had the best bacteriostatic effect at 48h, and its inhibitory effect on E.coli and S.aureus was significantly higher than that of Salmonella. 【Conclusion】 An entomopathogenic nematode strain with good reproductive ability and abiotic stress tolerance was obtained.The nematode had high pathogenicity to the Galleria mellonella larvae, and its symbiotic bacteria had good inhibitory effect on E.coli and S.aureus.