Abstract:
【Objective】 The objective of this study is to investigate the diversity of dsRNA in
A. solani., and to provide new biological control resources for the disease and new understanding of virus diversity and evolutionary.
【Methods】 Tissue samples with typical symptom were collected from tomato growing areas around Shihezi and pure culture was obtained by tissue isolation and single spore separation. After that, the diversity of dsRNA carried by
A. solani. was determined by dsRNA extraction and gel electrophoresis. In the end by RT-PCR using random primers, and molecular cloning, and sequencing techniques, the phylogenetics for the mycovirus carried by 6 strain were analyzed.
【Results】 The pure culture of 191 strains of
A. solani. was obtained and 42 strains with obvious dsRNA bands (from 1 to 4) were found. The mycovirus strains were accounting for 22% of the pure culture strains. The band's sizes were different among the dsRNA carried strains. The Contigs were obtained by sequence and assemble from 6 strains. The blast results showed that the 7 dsRNA virues and 2 +ssRNA virues were shared sequence homology with isolates among the
Totiviridae,
Partitiviridae,
Tymoviridae,
Tymoviridae, and(or)
Narnaviridae respectively.
【Conclusion】 There are diverse dsRNAs in
A. solani. The strains of
A. solani not only carry many mycoviruses, but also mixed infections with two or more unrelated viruses. The 9 mycovirues identified can be divided into 5 known families (
Totiviridae,
Partitiviridae,
Tymoviridae,
Tymoviridae, and
Narnaviridae). Some are reported viruses or new strains while others are new viruses that have not yet been discovered.