Abstract:
【Objective】 To study the types of virus diseases in potato in Xinjiang, China, in order to clarify the main virus pathogens of potato in this region and provide scientific basis for the scientific prevention China and control of potato virus diseases.
【Methods】 In 2021, 87 suspected susceptible potato virus samples were collected from Urumqi, Jimsar, Qitai, Balikun and Zepu counties in the main producing areas of Xinjiang, China,and RT-PCR was performed on the samples.CP genes of positive samples were cloned and sequenced, and homology, geographical origin and phylogenetic analysis were completed.
【Results】 Potato virus Y (PVY), Potato virus S (PVS) and Potato leaf roll virus (PLRV) were detected in 87 samples.The detection rates were 100%, 37.93% and 6.89%, respectively, with the highest detection rate of PVY, and no Potato virus X (PVX) and Potato virus M (PVM) were detected, indicating that PVY was the main virus pathogen harming Potato samples in this region.Cloning and sequence alignment of PVY positive disease samples were performed.The consistency of CP gene sequences of 5 samples with isolates from China(Fujian, Shandong, Jiangsu) and Poland reached 100%, and the consistency of CP gene sequences with isolates from other countries reached more than 99.04%.
【Conclusion】 Potatoes in Xinjiang,China main producing areas were mainly infected by one or more viruses of PVY, PVS and PLRV, among which PVY was the most serious pathogen.