鹰嘴豆种子代谢产物的GC-MS分析

Multivariable comparative analysis of chickpea seed metabolites based on GC-MS

  • 摘要: 【目的】 研究鹰嘴豆品种之间代谢物质的差异,利用 GC-MS技术对鹰嘴豆初级代谢产物进行代谢组学分析,为鹰嘴豆产品开发提供理论基础。 【方法】 利用GC-MS技术及热图聚类,比较分析10个鹰嘴豆品种种子的代谢产物。 【结果】 检测到146种化合物,主要包括:机酸类、脂肪酸类、氨基酸类、糖类、醇类、酚类以及含氮类七大类化合物。其中8个品种针对每一大类均含有相同的化合物,而D2016-10和YZD80则积累自身特定的化合物,与其他8个品种相同累积的化合物明显偏少。D2016-10和YZD80 2个品种所积累化合物情况完全不同,存在各自代谢产物的特异性。按化合物的Q值将10个鹰嘴豆品种分为4小类。 【结论】 10个不同鹰嘴豆品种均含有大量的酸类、糖醇类以及含氮类等几大类化合物,氨基酸类及其衍生物分布相对较少。

     

    Abstract: 【Objective】 To explore the differences of metabolites among different chickpea varieties by using GC-MS analysis combined with cluster analysis and to analyze their non targeted metabonomics, so as to provide a theoretical basis for the development of chickpea food, health products and drugs. 【Methods】 The metabolites of 10 chickpea seeds were analyzed by GC-MS. 【Results】 146 compounds were detected from the tested materials, mainly including organic acids, fatty acids, amino acids, sugars, alcohols, phenols and nitrogen-containing compounds. After cluster analysis, it was found that the all the eight varieties contained the same distribution of some compounds for each major category of compounds, but D2016-10 and YZD80 accumulated their own specific content of compounds, while the content of the same accumulated compounds of the other eight varieties was significantly less. However, the accumulation of these D2016-10 and YZD80 two varieties was completely different, so they had their own specificity. Divide 10 chickpea varieties into 4 subcategories based on their Q values of compounds. 【Conclusion】 In general, the 10 different chickpea varieties contain a large number of acids, sugar alcohols and nitrogen-containing compounds, and the distribution of amino acids and their derivatives is relatively small.

     

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