Abstract: 【Objective】 To study drip irrigation pipe distribution on biomass and nitrogen utilization efficiency and yield of machine-harvest Cotton,In the hope of laying a theoretical basis for high yield of machine-harvest Cotton. 【Methods】 We compared two kinds of drip irrigation pipe distribution: one was (66+10)cm with the three drip-irrigation belts inside the narrow row (G₃), and the other was (66+10)cm with the three drip-irrigation belts inside the narrow row and the two drip-irrigation belts inside the big row(G₅), and four nitrogen applications: 0 (N₀), 240 kg/hm² (N₂₄₀), 300 kg/hm² (N₃₀₀) and 360 kg/hm² (N₃₆₀). 【Results】 The results showed that the dry matter weight of G₅ treatment was significantly higher than the G₃ treatment under the same nitrogen application, compared with G₃-N₃₆₀ treatment, G₅-N₃₆₀ treatment the dry matter weight of leaves, stems, bolls and total increased respectively by 23.1%, 15.0%, 11.6% and14.9%.The nitrogen uptake of G₅ treatment was significantly higher than the G₃ treatment under the same nitrogen application, Compared with the G₃-N₃₆₀ treatment, the total nitrogen uptake of the G₅-N₃₆₀ treatment increased by 11.69%, compared with G₃-N₂₄₀ treatment, and the total nitrogen uptake of the G₅-N₃₀₀ treatment increased by 11.85%, compared with G₃-N₂₄₀ treatment, the total nitrogen uptake of G₅-N₂₄₀ treatment increased by 12.84%, the apparent nitrogen use efficiency of G₅ treatment was significantly higher than that of G₃-N₂₄₀ treatment, and the apparent nitrogen use efficiency of G₅-N₂₄₀ treatment increased by 3.6% higher than that of G₃-N₂₄₀ treatment, the apparent nitrogen use efficiency of G₅-N₃₀₀ treatment increased by 2.97% higher than that of G₃-N₃₀₀ treatment, and the apparent nitrogen use efficiency of G₅-N₃₆₀ treatment increased by 3.14% higher than that of G₃-N₃₆₀ treatment, the yield of cotton G₅-N₂₄₀ treatment increased by 10.6% higher than that of G₃-N₂₄₀ treatment, and the yield of cotton G₅-N₃₀₀ treatment increased by 8.3% higher than that of G₃-N₃₀₀ treatment, the yield of cotton G₅-N₃₆₀ treatment increased by 12.4% higher than that G₃-N₃₆₀ treatment. 【Conclusion】 Drip irrigation pipe distribution the G₅ treatment was superior to the G₃ treatment under the same nitrogen application rate, and the G₅-N₃₆₀ model had the highest on dry matter quality and bolls and nitrogen uptake and yield of cotton.