Abstract: 【Objective】 To analyze the effects of adding sodium selenite on the growth of fruiting body and agronomic traits of cultivated Morchella sextelata in the hope of providing theoretical and technical support for large-scale artificial cultivation of selenium-enrich Morchella. 【Methods】 The effects on different treatment of selenium concentrations on the yield and agronomic characters of Morchella sextelata were systematically analyzed by artificial cultivation with Morchella sextelata as selenium-enrich carrier and sodium selenite as exogenous selenium. 【Results】 When the selenium concentration was 0, the length of cap and the circumference of stipe were the longest. When the concentration of selenium was 10 mg/L, the perimeter and length of the cap were the longest, which could promote the growth of Morchella sextelata. When the concentration of selenium was 20 mg/L, the yield, dry weight per plant and total length of fruiting body of Morchella sextelata were the largest, which promoted the growth of Morchella sextelata. When the selenium concentration was 40 mg/L, the selenium concentration inhibited the growth of Morchella sextelata fruiting body. The results showed that the comprehensive evaluation score of various indexes of Morchella sextelata fruiting body cultivated under the concentration of selenium 20 mg/L was the highest, which was the best concentration. 【Conclusion】 Based onsodium selenite as exogenous selenium, 20 mg/L of selenium concentration is the best, which can be used as an ideal concentration for large-scale artificial cultivation of selenium-enrich Morchella.