Abstract: 【Objective】 This project aims to evaluate the metabolic potential of strain TRM SA0054 by analyzing the genomic information, thus further studying the secondary metabolite gene resources of the strain. 【Methods】 The whole genome of TRM SA0054 was sequenced by Illumina Hiseq sequencing platform, and bioinformatics analysis was conducted. The metabolites were preliminarily detected and identified by modern separation and purification methods. 【Results】 The genome size of strain TRM SA0054 was 7.2 Mb, there were 6,410 protein coding genes, and GC content was 73.16%. AntiSMASH analysis predicted that there were 26 secondary metabolic gene clusters, among which Region 36.1 gene cluster was 81% similar to Tubercidin gene cluster. Fermentation products were detected by LC-MS, the fermentation of strain TRM SA0054 could produce Tubercidin. 【Conclusion】 Strain TRM SA0054 has the ability to produce Tubercidin based on genome mining and LC-MS detection.