Abstract: 【Objective】 To investigate the effects of bio-organic fertilizer application on growth, yield, quality and soil properties of organic Lycium barbarum. 【Methods】 Ningqi no.7 was used as experimental material, and the effects of different bio-organic fertilizer treatments on the growth of Lycium barbarum L.and the differences of soil nutrient contents were compared during the growing season (July to November) with no fertilization as the control (CK). 【Results】 The results showed that compared with CK, application of bio-organic fertilizer decreased soil conductivity and pH, and increased soil organic matter, total nitrogen, total phosphorus, total potassium, alkali-hydrolyzable nitrogen, available phosphorus and available potassium contents.Application of 44.55 L/hm² compound bio-organic fertilizer had obvious soil improvement effect, which was significantly different from CK.After applying bio-organic fertilizer, the photosynthetic characteristics of Lycium barbarum leaves were improved, the tree height and crown width of Lycium barbarum plants were significantly increased, and 44.55 L/hm² compound bio-fertilizer had the best effect.After applying Trichoderma bioorganic fertilizer, compound bioorganic fertilizer and Bacillus bioorganic fertilizer, the yield of each treatment increased by 4.8%-21.7%, 11.9%-31.4% and 3.5%-21.7%, and the fruit quality was improved., and the fruit quality was the best when 44.55 L/hm² compound biofertilizer was applied per hectare. 【Conclusion】 After comprehensive evaluation, the growth status of Lycium barbarum under different bio-organic fertilizer treatments were improved to a certain extent, and the soil physical and chemical properties were improved, among which 44.55 L/hm² compound bio-organic fertilizer applied per hectare had the best effect.