Abstract:
【Objective】 In view of the major practical problem that paclobutrazole residue significantly inhibits the growth and development of leaf lettuce at seedling stage, a two-factor completely randomized trial design consisting of different leaf lettuce varieties and gibberellin mass concentrations was adopted to explore whether spraying gibberellin could alleviate the adverse effect of paclobutrazol.
【Methods】 A total of five leaf lettuce varieties and seven spraying levels with different mass concentrations of effective ingredient gibberellin, including A
1 (0 mg/L), A
2 (15 mg/L), A
3 (30 mg/L), A
4 (60 mg/L), A
5 (120 mg/L), A
6 (240 mg/L) and A
7 (480 mg/L), were carried out and the seedling quality, leaf type and the content of soluble solids in leaves of leaf lettuce were determined at the transplanting stage.
【Results】 With the increase of gibberellin mass concentration, all parameters of the leaf lettuce height, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight, total leaf area, total leaf length, total leaf width, maximum function leaf length, and maximum function leaf width of five cultivars were increased firstly and then decreased with the increase of gibberellin mass concentration.The values of all indexes reached the maximum at A
5 treatment, and compared with A
1 treatment, each index of A
5 treatment were increased by 36.8%-86.6%, 30.1%-53.4%, 21.1%-77.7%, 56.2%-127.3%, 56.0%-115.5%, 63.4%-142.1%, 30.4%-79.5%, 27.0%-65.7%, 24.0%-50.0%, 21.8%-48.8%, respectively.The indexes of soluble solids also showed a tendency to increase first and then decrease, with the best performance in A
2 andA
3 treatments.The SPAD value and root shoot ratio of leaf lettuce exhibited an opposite tendency and the minimum of all indexes was found in A
5 treatment.Compared with A
1 treatment, each index of A
5 treatment was significantly reduced by 17.6%-28.6%, 17.5%-36.2%, respectively.
【Conclusion】 In conclusion, the inhibition effect of paclobutrazol residue was alleviated by spraying gibberellin, and the gibberellins mass concentration A
5(120 mg/L) exhibited the best regulation effect on leaf lettuce seedlings.