Abstract: 【Objective】 Sea buckthorn fruits are usually harvested by freezing and cutting the fruiting branches before de-fruiting. To provide a basis for the development of sea buckthorn fruit harvesting and processing machinery, it is necessary to obtain the separating force of sea buckthorn fruits with different physical parameters at different freezing temperatures. 【Methods】 The SBT fruit "Late Autumn Red"was used as the experimental material.The characteristic parameters and other biophysical parameters of SBT fruits at different frozen storage temperatures were measured by digital explicit push-pull meter, digital vernier caliper, electronic scale and texture analyzer.Meanwhile, the experimental data were analyzed by variance analysis and correlation analysis. 【Results】 With the increase of SBT tree age, the fruit detachment force, fruit stem diameter, fruit longitudinal and equatorial diameter, fruit mass increased significantly.With the decrease of the frozen storage temperature, the pericarp strength increased 16.86g/mm², and the fruit drop hardness decreased by 42.05g.The fruit detachment force was positively correlated with fruit stem diameter, fruit longitudinal and equatorial diameter, fruit mass (P<0.01); fruit mass was positively correlated with fruit detachment force, fruit stem diameter, fruit longitudinal and equatorial diameter (P< 0.01).There was a significant negative correlation between pericarp strength and fruit drop firmness (P< 0.01). 【Conclusion】 SBT fruit with older tree age and lower frozen storage temperature is more suitable for harvest, and the fruit detachment force, fruit mass and pericarp strength are highly correlated with other parameters, thus providing some index reference for mechanical selective harvest.