Abstract: 【Objective】 To study the physiological changes of 42 creeping peanut varieties under salt stress. 【Methods】 Hydroponic method was used to measure the physiological indexes of proline (Pro), malondialdehyde (MDA), chlorophyll (Chl) and soluble sugar in the leaves of seedlings under the short-term stress concentration of 200 mmol/LNaCl.The comprehensive analysis was carried out by membership function method in order to provide a basis for screening salt-tolerant peanut varieties. 【Results】 (1) The proline (Pro) content of peanut leaves increased in seedling stage;(2)The content of malondialdehyde (MDA) increased except for 16 varieties c095, Z5036, Z5081, C094, C108, C128, C141, C145, C145-2, Z5096, Z5108, Z5123, Z5157, Z5185, Z5230, C108-1, and other varieties showed a downward trend and good salt tolerance response.(3) The chlorophyll (Chl) content of 80% peanut varieties increased compared with CK, while the contents of C905, Z5036,Z5040, C035, C087, C129, C135, Z5083, Z5225 decreased.(4) Compared with CK, most of the soluble sugar content showed an upward trend, and only the chlorophyll content of Z5035, C001, C087, C128, C135, C141, C145, C145-1, C145-2, Z5028, Z5123, Z5157,Z5225 decreased, which belonged to salt sensitive varieties. 【Conclusion】 Cluster analysis is used to classify 42 creeping peanut varieties into four salt tolerance groups, namely, high salt tolerance group, salt tolerance group, medium salt tolerance group and salt sensitive group.