Abstract: 【Objective】 This project aims to screen and analyze the UGT gene family of cotton glycosyltransferase damaged by Helicoverpa armigerawere in the hope of providing a basis for in-depth researches into the roles of UGTG01, UGTG02, UGTGo4, UGTGo6, and UGTGo7 genes. 【Methods】 Based on the genome database of cotton glycosyltransferase UGT damaged by Helicoverpa armigera, the bioinformatics method was used to systematically analyze the physical and chemical properties, phylogenetic tree, secondary structure prediction, conserved motif, and conserved domain of cotton glycosyltransferase UGT gene family. 【Results】 Five putative members of the UTG gene family with amino acid numbers ranged from 468 to 534, whose theoretical isoelectric point distributions of was from 4.97 to 5.58, relative molecular mass ranged between 52,743.1 and 60,208.62, subcellular localization outside the cytoplasm, and hydrophilicity as proteins were discovered through a genome search of cotton. The gene structure contained one exon, and the secondary structure was mainly composed of α-helix, irregularly coiled, with three motifs and a Glycosyltransferase_GTB-type superfamily domain. Expression analysis showed that the five genes displayed notable variations in the root, stem, cotyledon, true leaf, flower, and cotton boll; molecular evolutionary tree exhibited that the five UGTs were found to be on the same branch of the UGT73C family genes, rice UGT707A3 and soybean GmUGT. 【Conclusion】 These glycosyltransferase genes have markedly varied levels of expression in various tissues and are found to be on the same evolutionary branch as UGT73C, UGT707A3, and GmUGT in evolutionary tree analysis, which indicates that they may have similar insect resistance functions, and also respond to insect resistance in cotton by regulating saponin elements, flavonoids, and naringenin.