Abstract: 【Objective】 In order to explore the regulation effect of 0.1% thiabenduron combined with different exogenous substances on cotton growth, development, yield and quality. 【Methods】 A field experiment was conducted in Dunlimai village, Hailou Township, Shaya County in 2021.Xinluzhong 67, mainly 0.1% thiabenduron 150 g / hm², was mixed with 14 hydroxybrassinolate 150 g / hm² (TDZ₁), sodium dinitrophenol 300 g / hm² (TDZ₂), prochloraz 150 g / hm² (TDZ₃), inducer 300 g / hm² (TDZ₄) and ethephon 300 g / hm² (TDZ₅), with clear water as the control (CK), The effects of 0.1% thiabenduron combined with different exogenous substances on agronomic traits, dry matter accumulation and distribution, yield and quality of cotton were analyzed. 【Results】 TDZ₁, TDZ₂ and TDZ₅ increased by 0.66, 0.33 and 1.53 compared with the control, and the number of buds and bolls increased by 8.68%, 17.23% and 23.45% compared with the control; Compared with the control, the weight of reproductive organs of TDZ₅ increased by 1.93%.Each treatment increased the single boll weight and yield of cotton, among which, TDZ₂ and TDZ₃ increased by 1.53 g and 1.28 g, and TDZ₁ and TDZ₃ increased by 31.82% and 29.50% compared with the control.The elongation of TDZ₃ was higher than that of the control by 1.04%.The number of fruit branches was significantly positively correlated with plant height, stem diameter and number of buds and bolls, the number of buds and bolls was significantly positively correlated with plant height and stem diameter, the number of buds and bolls per plant was positively correlated with stem diameter, number of buds and bolls per plant, and the yield was significantly positively correlated with the number of bolls per plant and single boll weight. 【Conclusion】 Spraying 150g/hm² 0.1% thiabenduron and 150g/hm² 14 hydroxybrassinolate can increase the number of buds and bolls, the number of bolls per plant and the weight of bolls per plant, thus significantly improving the yield.