Abstract: 【Objective】 To discover candidate genes related to drought resistance in the hope of providing theoretical basis for subsequent identification and identification and evaluation of drought resistance of cotton and research on drought resistance genes. 【Methods】 In this experiment, 20 extreme drought and drought sensitive materials selected from recombinant inbred lines were constructed from Upland Cotton Shiyuan 321 and Kui 85-174.The extreme drought and extremely sensitive drought pools were constructed respectively, BSA sequencing was analyzed with the parents, and candidate genes were mined by BSA sequencing analysis using the upland cotton genome as a reference. 【Results】 The results showed that they were finally associated with 3 related candidate regions.The total length of the candidate regions was 6.12 Mb, which contained a total of 86 genes.Combining GO annotation, KEGG pathway and five key indicators selected in the previous stage, through qRT-PCR detection and analysis, a total of 5 candidate genes were finally determined. 【Conclusion】 The expression levels of the five genes in leaves were significantly higher than those in roots and stems.And the expression levels in drought-resistant materials were all higher materials with weak drought resistance, and the overall trend was “rising first and then falling”.