Abstract:
【Objective】 To develop KASP (Kompetitive Allele Specific PCR) marker for efficiently identifying CR (clubroot resistance) genes in cabbage
B.rapa germplasms by using KASP markers to explore the distribution of clubroot resistant genes in germplasm collected with a large number of
B.rapa accessions.
【Methods】 Based on the information of cloned clubroot resistance genes previously, KASP markers were developed using Primer 3 and DNAMAN and the highly reliable markers were screened using a representative population containing 62
B.rapa varieties.Then, these available markers were used to genotype the 862
B.rapa resource population for disease resistance.
【Results】 Based on four cloned disease resistance genes (
CRa,
CRb,
CRd,
Crr1
a), six gene-specific KASP markers related to clubroot disease resistance were developed and screened.Among the 862
B.rapa germplasm accessions, 105 accessions containing clubroot resistance genes were identified.Turnip and turnip rape accounted for 70.48%.
CRa was found to be the clubroot resistance gene carried by most accessions among
B.rapa germplasm resources, which accounted for 58.09% of all disease resistant materials.Additionally, we found 16 accessions that contained two or more resistance genes.
【Conclusion】 In this study, six stable and reliable gene-specific KASP markers were developed.
CRa was found to be the clubroot resistance genes carried by most accessions among the four CR genes.Importantly, a few germplasm resources were identified that carried multiple clubroot resistance genes.