新疆拜城县马铃薯疮痂病病原菌的分离鉴定及生物学特性分析

Isolation, identification and biological characteristics of potato scab pathogen in Baicheng, Xinjiang

  • 摘要: 【目的】 研究新疆阿克苏地区拜城县马铃薯疮痂病病原菌的种类。 【方法】 采集新疆阿克苏地区拜城县3个不同种植区的马铃薯疮痂病块茎及薯表土壤,采用组织分离法和稀释涂布分离法,并通过致病性测定、形态学、分子生物学及生理生化特性对病原菌进行鉴定。 【结果】 在分离出的72株放线菌中,菌株K6含致病基因txtAB、 tomA、 nec1且对萝卜幼苗有较强致病性;经鉴定为同一种链霉菌属Streptomyces bobili,为新疆首次报道的病原菌。所有供试碳源、氮源均可作为单一碳源、氮源被菌株S.bobili K6利用,在酪氨酸琼脂培养基上有黑色素产生,对6%NaCl、0.1%苯酚、20 μg/mL链霉素敏感,在pH4的条件下不能正常生长。 【结论】 新疆拜城县马铃薯疮痂病病原菌为S.bobili,包含3种致病基因txtABtomAnec1,且致病性较强。

     

    Abstract: 【Objective】 To clarify the species of potato common scab pathogenic bacteria in Baicheng County, Aksu Prefecture, Xinjiang. 【Methods】 Potato common scab tubers and potato surface soil were collected from three different growing areas in Baicheng County, Aksu Prefecture, Xinjiang, and the pathogenic bacteria were identified by tissue isolation method and dilution coating isolation method, and by pathogenicity determination, morphology, molecular biology and physiological and biochemical characteristics. 【Results】 Among the 72 isolated actinomycetes, six strains contained the pathogenic genes txtAB, tomA and nec1 and were strongly pathogenic to radish seedlings; they were identified as the same Streptomyces bobili, which was the first reported pathogen in Xinjiang.All the tested carbon and nitrogen sources were available as single carbon and nitrogen sources by strain S.bobili K6, with melanin production on complex agar medium, sensitive to 6% NaCl, 0.1% phenol, 20 μg/mL streptomycin, and not growing any more at pH 4 normally. 【Conclusion】 The pathogenic of potato common scab is S.bobili in Baicheng County of Xinjiang, which contains three pathogenic genes txtAB, tomA, and nec1, and belongs to highly pathogenicity.

     

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