Abstract:
【Objective】 To clarify the species of potato common scab pathogenic bacteria in Baicheng County, Aksu Prefecture, Xinjiang.
【Methods】 Potato common scab tubers and potato surface soil were collected from three different growing areas in Baicheng County, Aksu Prefecture, Xinjiang, and the pathogenic bacteria were identified by tissue isolation method and dilution coating isolation method, and by pathogenicity determination, morphology, molecular biology and physiological and biochemical characteristics.
【Results】 Among the 72 isolated actinomycetes, six strains contained the pathogenic genes
txtAB,
tomA and
nec1 and were strongly pathogenic to radish seedlings; they were identified as the same
Streptomyces bobili, which was the first reported pathogen in Xinjiang.All the tested carbon and nitrogen sources were available as single carbon and nitrogen sources by strain
S.bobili K6, with melanin production on complex agar medium, sensitive to 6% NaCl, 0.1% phenol, 20 μg/mL streptomycin, and not growing any more at pH 4 normally.
【Conclusion】 The pathogenic of potato common scab is
S.bobili in Baicheng County of Xinjiang, which contains three pathogenic genes
txtAB,
tomA, and
nec1, and belongs to highly pathogenicity.