Abstract: 【Objective】 To evaluate the effects of different microbial treatments on tomato soil microbial diversity, and provide theoretical basis and reference for improving soil microbial diversity and increasing tomato yield. 【Methods】 With fruit tomato Nongbo powder 18109 as the test material, high-throughput sequencing technology was used to study the effects of microbial bacteria on tomato soil microbial diversity and environmental relevance of one control (CK) treatment and three microbial bacteria treatments of Actinomycetes (T₁), Bacillus subtilis (T₂) and Trichoderma harzianum (T₃). 【Results】 T₃ had the highest bacterial content, T₁ had the highest fungal content.The ratio of bacteria to fungi in T₁ and T₃ was greater than that of the CK, and soil microorganisms presented "bacterialization".Alpha diversity showed that T₁ and T₃ had more bacterial species than CK.T₁, T₂, and T₃ have fewer fungal species than CK.Shannon and Simpson indexes of bacteria treated with T₂ were lower than those of CK, and the Shannon and Simpson indexes of bacteria treated with T₁ and T₃ were lower.The Shannon and Simpson indexes of the fungi treated by the three microorganisms were higher than that of CK.There were differences among the four treatments of control, Actinomycetes, Bacillus subtilis, and Trichoderma harzianum, and there was a strong consistency among the same treatments.The bacterial and fungal diversity of T₁ and T₃ microbial treatments were similar.Among the bacteria, Unspecified_iii1_15,Unspecified_RB41, Unspecified_Gemm_5, Unspecified_Syntrophobacteraceae, Kaistobacter were the dominant bacterial genera.The relative abundance of Kastobacter in CK, T₁, and T₃ was the highest, and in T₂ the relative abundance of Bacillus was the highest.Among the fungi, Fusarium, Unspecified_Chaetomiaceae, Unspecified_Pezizales, Unspecified_Hypocreales_fam_Incertae_sedis, Mortierella were the dominant fungi, the relative abundance of Fusarium was the highest among the 4 different treatments.The correlation between leaf width and microbial community distribution in bacteria and fungi was the smallest, and the correlation between leaf length, plant height, stem thickness, yield and microbial community distribution was high.The effect of Actinomycetes and Trichoderma harzianum on tomato soil microorganisms was relatively strong.The correlation between leaf length, leaf width, stem thickness and Actinomycetes was the strongest, and the correlation between yield and Trichoderma harzianum was the strongest. 【Conclusion】 Different microbial treatments have different effects on tomato soil microbial diversity, but Actinomycetes and Trichoderma harzianum havw relatively greater effects on tomato soil microorganisms, and leaf length, leaf width and stem diameter have the highest correlation with Actinomycetes, Trichoderma harzianum has the highest correlation with yield.