Abstract: 【Objective】 Study EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot. 【Methods】 In this study, different concentrations of ethyl methanesul fonate (EMS) were employed on wheat seeds of cultivar Hengguan 35.The untreated Hengguan 35 was used as the susceptible control, and Shiyou 17 was used as the moderately resistant control.Through artificial inoculation of Fusarium pseudograminearum, the resistance of the mutagenized material was identified, and the M₃ generation material resistant to FCR was obtained by adding generation in the greenhouse. 【Results】 The EMS concentration suitable for Hengguan 35 mutation was finally determined to be 0.4%, and seven mutants showing immunity at the plant stage were obtained from 106 M₃ Hengguan 35 materials.The overall disease index of the mutant materials at seedling and adult stages was 34.93% and 34.62% lower than those of the susceptible control, respectively. 【Conclusion】 After EMS mutation of wheat Hengguan 35, an appropriate mutation concentration of 0.4% was determined. A total of 7 immune resistant wheat FCR were screened from the M3 generation of the tested mutant varieties.