Abstract: 【Objective】 The aim is to analyze the biological characteristics and chemical constituents of Inonotus obliquus in Xinjiang. 【Methods】 A strain HS819, which was isolated from sclerotium was collected from Altay, Xinjiang.The carbon source utilization patterns of the strain were characterized by Biolog FF microplate.Chemical components of the nucleus, mycelium and fermentation broth detected in mineral ether were extracted and analyzed by using GC-MS. 【Results】 The result of the ITS blast showed that the similarity between strain HS819 and Inonotus obliquus strain PAT29027 (OP019327) was 99.73%.And the strain was identified as Inonotus obliquus by the morphological characteristics of sclerotium, colony, hyphae and phylogenetic analysis.Strain HS819 had the strongest metabolic ability to seven carbon sources, such as D-ribose, glycyl-L-glutamic acid, salicin, β-methyl-D-galactoside, α-keto-glutaric acid, D-cellobiose, and L-glutamic acid.There were 43, 39 and 38 different chemical components detected in sclerotium, mycelium and fermentation broth by using GC-MS, respectively.The relative content of hydrocarbons, acids and lipids was significantly higher than that of aldehydes and alcohols, of which acids were the main components of sclerotium and mycelium, accounting for 66.07% and 60.03% of the total components, respectively, and hydrocarbons were the main components in fermentation broth, accounting for 54.01%. 【Conclusion】 Cellulose and hemicellulose are the main components in carbon source utilization to strain HS819.There are significant differences in the types and contents of chemical components detected in the natural environment and the liquid fermentation process of the strain.The differential expression of genes may be related to biological stress and growth environment in the two states.