Abstract: 【Objective】 To study the effect of sulfur dioxide (SO₂) on sugar content and sugar metabolism pathway in Munage grape fruit. 【Methods】 The changes of sucrose, glucose and fructose contents in Munage grapes during storage were determined by high performance liquid chromatography (HPLC) and simultaneously, the expression differences of 20 genes related to glucose metabolism were evaluated. 【Results】 SO₂ treatment significantly increased the glucose content from 0 to 10 days of storage.On the 60th day of storage, the contents of sucrose, glucose and fructose in the SO₂-treated grape were increased to varying degrees compared with that of the control group.SO₂ up-regulated cell wall invertase (CWINV), neutral/alkaline invertase (CIN), sucrose synthase (SuSy) and sucrose phosphate synthase1 (Sucrose phosphate synthase1, SPS1) gene expression level to maintaine high sugar content in grape during storage.Moreover, SO₂ activated the photosynthetic activity of grape by inducing the expression of hexokinase 2 (HxK2), fructose-bisphosphate Aldolase (FBA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK). 【Conclusion】 SO₂ can regulate the expression levels of related genes to modulate the glucose metabolism-related pathways in the postharvest storage of Munage grapes.