Abstract: 【Objective】 To study the effects of different scions on the survival rate of grape grafting and root characteristics of rootstock and lay a foundation for selecting suitable rootstock-scion combinations. 【Methods】 Three different rootstocks including 5BB, 1103P and Shanhe 1 were branch grafted with scions including Hutai 8, Red Globe and Suxin 1, respectively.The survival rate of each combination was counted and the root growth, physiological and biochemical characteristics and the anatomical structure of root tips was observed by paraffin section method. 【Results】 Among the nine rootstock and scion combinations, the four rootstock-scion combination with higher grafting survival rate were Suxin 1/ Shanhe 1, Red Globe/ Shanhe 1, Suxin 1/5BB and Hutai 8/Shanhe 1, which were 74.33%, 65.67%, 63.33% and 60.00% respectively.while the graft survival rate of Hutai 8/5BB, Hutai 8/ 1103P and Red Globe/ 5BB combinations were all lower than 40.00%.The root number of Suxin 1/ Shanhe 1 rootstock-scion combination was the highest in three different soil layers, and Hutai 8/Shanhe 1 rootstock-scion combination was the second; Compared with the rootstock-scion combinations of Red Globe/ 1103P, Red Globe/ 5BB and Hutai 8/ 1103P, the root activity, soluble protein content, soluble sugar content and SOD enzyme activity of the grafted seedlings of Suxin 1/ Shanhe 1 rootstock combination were significantly higher than those of these rootstock-scion combinations.Different scions had different effects on the diameter, area, density, xylem area and stele area of rootstock root system.The results of correlation analysis showed that except the relative conductivity index, the other indexes were significantly correlated with the grafting survival rate. 【Conclusion】 Different scions have effects on the survival rate, root morphological characteristics, physiological characteristics and root tip anatomical structure of rootstocks.Through comprehensive analysis, Shanhe 1 can be used as rootstock for Suxin 1, Hutai 8 and Red Globe, 5BB can be used as rootstock for Suxin 1, and the best rootstock-scion combination is Suxin 1/Shanhe 1.