Abstract: 【Objective】 To study the response of quinoa at germination stage to low temperature stress, to evaluate its low temperature tolerance by using multiple germination indexes, and to analyze the physiological characteristics of different quinoa varieties at different germination temperatures, so as to provide theoretical basis for planting quinoa at suitable germination stage in production. 【Methods】 6 quinoa varieties with good adaptability in Yili River Valley of Xinjiang were taken as test materials and the germination test was performed in 1℃, 5℃, 10℃, 15℃ and 20℃ incubators to measure the germination potential, germination rate, germination index, vigor index, bud length and bud fresh weight of each quinoa variety at different temperatures, and the six quinoa varieties were comprehensively evaluated under different temperature conditions by means of difference analysis of different indexes, correlation analysis of relative germination indexes, principal component analysis and membership function analysis. 【Results】 With the decrease of temperature, the values of all germination indexes decreased, and there were significant differences among different quinoa varieties under different temperatures, and there was a certain correlation between the relative values of the six germination indexes. The characteristic value of factor 1 was 4.855, accounting for 80.924% of the total information. The characteristic values of other principal component factors were all less than 1, so the relative germination potential, relative germination index and relative vigor index could be used as the main identification indexes of low temperature tolerance of quinoa during germination stage. 【Conclusion】 YN₁ and YN₃ varieties are the most sensitive to low temperature at germination stage, and not suitable for early sowing. Varieties YN₂, YN₅ and YN₆ show stable performance in all aspects under different temperatures. Although their germination speed are slower at low temperature, but the germination rate are high and the low temperature tolerance is strong, belonging to the low temperature tolerance varieties. YN₄ cultivar has the most outstanding performance under low temperature condition and belongs to extremely low temperature tolerance cultivar.