Abstract: 【Objective】 To clarify the effects of different bio-organic fertilizers application combined with conventional fertilization on traits, yield and economic benefit of soybean plant. 【Methods】 Dry matter, root nodule, disease resistance and yield were studied by plots field experiment in which different bio-organic fertilizers combined with conventional fertilization were determined. 【Results】 Compared with conventional fertilization, aboveground dry matter, root dry weight and nodule number of soybean increased in different bio-organic fertilizer application treatments at seedling and full flowering stages by 5.31%-12.86%, 10.35%-20.24% and 12.34%-37.50%, respectively (P<0.05).Incidence rate and disease index of root rot were reduced significantly at the seedling stage in bio-organic fertilizer application treatment.Meanwhile, plant height, pod number per plant, grain number per plant and 100-grain weight of soybean were remarkably increased, which led to an improvement of soybean yield.The yields in 2019 and 2020 were lifted by 7.25%-8.67% and 6.50%-8.82%, respectively (P<0.05).Output and benefit of soybean were raised by applying bio-organic fertilizers combined with conventional fertilization compared with the control and the highest benefit addition value was 1,066.16 yuan/hm². 【Conclusion】 By applying bio-organic fertilizers combined with conventional fertilization, the growth of soybean is promoted, diseases are reduced, yield is promoted and economic benefit is increased.