Abstract: 【Objective】 To investigate the expression of hypoxia inducible factor (HIF) and its downstream target gene erythropoietin (EPO) and its receptor (EPOR) in ovine tissues under normal physiological conditions.The results have provided a histological basis for the study of targeted regulation or alleviation of ovine hypoxia stress. 【Methods】 The relative expression of HIF, EPO and EPOR genes in 14 tissues of 4 Xinjiang fine wool sheep were detected by real-time fluorescence quantitative PCR. 【Results】 HIF-1α and HIF-2α the relative expression of genes in ovine lung tissues were the highest and significantly higher than those in other tissues (P< 0.01); HIF-2α in lung the gene expression was about HIF-1α 5.2 times.The relative expression of EPO gene in ovine kidney was the highest and significantly higher than that in pituitary, colon, spleen, cecum, liver, adrenal gland, rumen, hypothalamus and heart (P< 0.05).The expression of EPOR gene in lung was the highest, and the relative expression of lung and testis was significantly higher than that in spleen, hypothalamus, kidney and heart (P< 0.05). 【Conclusion】 HIF-1α, HIF-2α and EPO and its receptor genes are highly expressed in the lung or kidney of sheep, suggesting that these two may be important organs for ovine hypoxia stress perception and regulation.