Abstract: 【Objective】 Investigation of brucellosis (brucellosis) in grazing sheep, the applicability analysis of testing methods, and the implementation of prevention and control measures. 【Methods】 90 serum samples from aborted sheep were detected by RBT, GICA, SAT, MAT, FPA, iELISA, cELISA and PCR, and the applicability characteristics of the diagnostic method and the results of the field investigation were used for statistical analysis, and control measures were proposed. 【Results】 In 90 sheep serum samples, positive RBT, GICA, SAT, MAT, FPA, iELISA and cELISA detection rates were 8.89%(8/90), 12.22%(11/90), 17.78%(16/90), 14.44%(13/90), 21.11%(19/90), 25.56%(23/90), 24.44%(22/90) and 14 positive samples by PCR. 【Conclusion】 High sensitivity methods were used for initial screening and strong specificity method were used for diagnosis in clinical practice.To be more specific, RBT, GICA, FPA or iELISA could be used for initial screening, and SAT, MAT or cELISA for diagnosis.The test results showed that the positive rate of grazing sheep increased with age (adult sheep 42% > weaning lamb 2.5%).A higher positive rate were detected in females (29.68%) than in males 11.54trading and slaughter were allowed%).Sheep with miscarriage history had a higher risk of brucellosis and adult females were more susceptible to brucella.The positive sheep were treated innocuously, the sheep shed and the surrounding environment were thoroughly disinfected, the negative sheep were immunized using the brucellosis M5-90 weak toxicity vaccine, and two months after immunization they were allowed to be slaughtered or for trading.