Abstract: 【Objective】 To determine the resistance of upland cotton germplasm resources to Fusarium oxysporum wilt specific type (Fusarium oxysporumf. sp.Momordicae) race 7, and to optimize the identification method and evaluation of disease resistance at seedling stage, so as to provide basic resistance source for cotton disease resistance breeding. 【Methods】 Using 265 upland cotton were taken as test materials, and the resistance of cotton to Fusarium wilt at seedling stage was identified by root irrigation method.; Xinjiang Junmian No.1 was used as the susceptible control variety, and the disease grade was recorded by investigation. After that, the relative disease index was used to evaluate the type of resistance. 【Results】 The Fusarium wilt resistance of 265 upland cotton germplasm resources was evaluated.The results showed that there were 5 varieties (lines) with high resistance to (I), 78 varieties (lines) with resistance to (R), 130 varieties (lines) resistant to (T) and 52 varieties (lines) with susceptible (S) characteristics, which were not immune to Fusarium wilt, 5 varieties (lines) with high resistance to (HR), 78 varieties (lines) with high resistance to Fusarium wilt, 130 varieties (lines) with disease resistance and 52 varieties (lines) with susceptible (S) characteristics.The percentages in the total number of Fusarium wilt resistance identified varieties (lines) were 1.9%, 29.4%, 49.1% and 19.6%, respectively. 【Conclusion】 Through the identification of Fusarium wilt resistance of upland cotton resource materials at seedling stage, it was found that the most resistant varieties (lines) were Fusarium wilt resistant varieties (lines), followed by disease resistant varieties (lines).These findings provide a germplasm resource with definite resistance for cotton breeding for Fusarium wilt resistance in the future.