Abstract: 【Objective】 In order to study the differences of amino acid content and composition of different seabuckthorn varieties, 15 seabuckthorn varieties in Qinghe County were studied, and the amino acid types and content were detected quickly by amino acid automatic analyzer. 【Methods】 The amino acid contents of 15 Seabuckthorn fruits were comprehensively evaluated by correlation, principal component analysis and cluster analysis. 【Results】 There were relative independence and close correlation among 15 quality indexes of seabuckthorn fruit; According to the comprehensive score of principal factors of principal component analysis, the top 5 seabuckthorn varieties ranked by 15 seabuckthorn varieties were BT-06-04, QH-03-02, BT-08-02, HK-01-01 and proposed QH-03-03, followed by late autumn red, anjr and HH-09-01. 【Conclusion】 Three principal components were extracted by principal component analysis, and the cumulative variance contribution rate was 88.296%. A comprehensive evaluation model was obtained: F=0.802F₁+0.122F₂+0.076F₃, which better reflected the comprehensive information of amino acids in seabuckthorn fruit. Fifteen Seabuckthorn fruits were divided into three categories by systematic cluster analysis. The cluster results were consistent with the results of principal component analysis, which better reflected the differences among different germplasm of seabuckthorn fruits.