Abstract: 【Objective】 In this study, the influence of different detection methods on the determination results of maize amylose and amylopectin content was discussed to provide a theoretical basis for the detection of maize starch content. 【Methods】 The contents of amylose and amylopectin in different maize were determined by dual wavelength spectrophotometer. The reference wavelength, absorbance and absorption spectrum of starch solution in different maize were compared, and the starch granule structure was observed by scanning electron microscope to study the factors affecting the determination of corn starch content. 【Results】 The amylose content in high amylose corn, normal corn and waxy corn is 64.8%, 25% and 1.9% respectively, and the amylopectin content is 32.3%, 75.6% and 97.1% respectively. Under acetic acid and hydrochloric acid treatments, the pH values of the three corn starch solutions decreased, and acetic acid had the greatest effect on the pH values of high amylose corn starch solutions. 【Conclusion】 Under hydrochloric acid treatment, the absorbance values and absorption peaks of the three starch solutions were higher than those of acetic acid treatment, and the absorbance and absorption spectra of amylose were higher than those of amylopectin. Normal corn starch granules are swollen, uneven and irregular in shape. Although the structure of high amylose corn starch granules has changed, it is not as violent as normal corn. Amylose aqueous solution is not stable as amylopectin aqueous solution.