Abstract: 【Objective】 The aim of this study is to establish an oxidative and inflammatory model of porcine jejunal epithelial cells (IPEC-J2) induced by lipopolysaccharide (LPS), and explore the anti-inflammatory and anti-oxidation mechanism of helicid (HEL). 【Methods】 The experiment was divided into 5 groups: control group, LPS group, HEL (25, 50 and 100 μM) + LPS group. 【Results】 The results showed that HEL could significantly reduce the expression of TNF-α, IL-1β and IL-6 at mRNA level, and we further verified the anti-inflammatory mechanism of HEL at protein level by ELISA.In terms of oxidative damage, LPS could significantly improve the expression of oxidation related proteins (Nrf2 and HO-1) and the activity of antioxidant enzymes (SOD and CAT), and significantly reduce the content of oxidation products (MDA).Importantly, HEL showed the opposite results, reducing the expression of Nrf2, HO-1 and the activity of SOD and CAT significantly and also significantly increasing the content of MDA. 【Conclusion】 In conclusion, all the results show that HEL can alleviate the oxidative and inflammatory damage of IPEC-J2 cells caused by LPS, and protect cells from injury to a certain extent.