Abstract: 【Objective】 The objective of this research is to investigate the effects of different nitrogen and phosphorus on the photosynthetic fluorescence of Jujube in the hope of providing theoretical guidance for industrial fertilization management of jujube. 【Methods】 In this study, seven consecutive 4-year jujube from the 13th Regiment of Alar City was taken as the research object in 2020.A two-factor completely random block experiment design was adopted to set up 10 fertilization treatments to analyze the difference of the influence of different fertilizer treatments on photosynthetic fluorescence. 【Results】 Compared with the control N₀P₀ (No nitrogen and phosphate were applied) treatment, Fm, Fo, Qp, Fv/Fm, Y(II), photosynthetic rate and water utilization ratio of jujube leaves were increased and intercellular CO₂ concentration and evaporation rate were also significantly decreased under each fertilizer treatment.N₁P₃ (202.5 kg/hm² for pure nitrogen and 540 kg/hm² for phosphorus pentoxide) and N₂P₃ (405 kg/hm² for pure nitrogen and 540 kg/hm² for phosphorus pentoxide) treatments were more beneficial to the improvement of net photosynthetic rate and water utilization ratio of jujube leaves.N₁P₃ (202.5 kg/hm² for pure nitrogen and 540 kg/hm² for phosphorus pentoxide), N₂P₂ (405 kg/hm² for pure nitrogen and 375 kg/hm² for phosphorus pentoxide) and N₂P₃ (405 kg/hm² for pure nitrogen and 540 kg/hm² for phosphorus pentoxide) treatments could effectively reduce the transpiration rate of jujube leaves.N₁P₂ (202.5 kg/hm² for pure nitrogen and 375 kg/hm² for phosphorus pentoxide) and N₂P₂ (405 kg/hm² for pure nitrogen and 375 kg/hm² for phosphorus pentoxide) treatments showed better performance in reducing intercellular CO₂ concentration. 【Conclusion】 Based on the different effects on photosynthetic fluorescence, the combination of N₂P₃ (405 kg/hm² for pure nitrogen and 540 kg/hm² for phosphorus pentoxide) nitrogen and phosphorus was more beneficial to improve the photosynthetic rate of jujube leaves.